U. Na-nakorn et al., Isolation and characterization of microsatellite loci of Clarias macrocephalus and their application to genetic diversity study, FISHERIES S, 65(4), 1999, pp. 520-526
Four microsatellite loci were identified for Clarias macrocephalus and the
forward and reverse primers for each locus were designed according to their
unique flanking regions. One locus, Cma-1*, was monomorphic, three loci, C
ma-2*, Cma-3* and Cma-4*, were polymorphic having 5, 14 and 30 alleles per
locus respectively. The polymorphic microsatellite loci were studied in fou
r natural populations of C. macrocephalus collected from different location
s in Thailand. Mean number of alleles per locus were 8.0, 8.7, 6.0 and 10.0
, respective to populations from Pattani, Pattalung, Chiangrai and Prachinb
uri. Mean heterozygosities were 0.744, 0.765, 0.718 and 0.810 respectively.
Nei's genetic distance showed the closest relationship between Pattalung a
nd Prachinburi (0.230) and the largest genetic distance between Pattalung a
nd Chiangrai (0.535). However, the genetic distances obtained in this study
were not accorded with geographic distance.