Differential expression of cyclooxygenase 2 in human colorectal cancer

Background-Experimental, clinical, and epidemiological studies have implica ted mitogenic metabolites of arachidonic acid such as prostaglandin E-2 (PG E(2)) in colorectal carcinogenesis. Recently, cyclooxygenase 2 (COX-2) whic h catalyses the conversion of arachidonic acid to PGE(2), has displayed inc reased levels in human colorectal cancer. Aims-To evaluate whether there is differential COX-2 expression from differ ent locations (caecum, ascending, transverse, descending, or sigmoid colon, and rectum) in human colorectal cancer. Methods-Protein levels of COX-2 were determined by western blot analysis in tumours and adjacent normal mucosa of 39 patients with colorectal cancer. Results-There was a notable overexpression of COX-2 protein in tumours loca ted in the rectum (p<0.001) compared with other locations in the colon. Rec tal tumours revealed elevated COX-2 protein levels in 18/20 cases compared with 4/19 colonic cases. No association between enhanced COX-2 protein expr ession in tumour tissue and Dukes's stages was found, Conclusions/Results suggest that the differential COX-2 expression may be d ue to differences in gene regulatory factors affecting COX-2 expression and /or reflect secondary changes in tumour progression which may have clinical implications.