Phenotypic changes in neutrophil granulocytes from healthy donors after G-CSF administration

Background and Objectives. The aim of the present work was to analyze the i mmunophenotypic changes in neutrophil granulocytes (NG) and their evolution over time in 16 healthy donors who received G-CSF for stem cell mobilizati on for allogeneic peripheral blood stem cells (PBSC) transplantation. Design and Methods. The mobilization schedule consisted of G-CSF (Filgrasti m(R), Amgen) 10 mu g/kg, s.c. for five consecutive days. Apheresis began 16 -24h after the last dose of G-CSF. Immunophenotypic analysis (CD10, CD14, C D15, CD16, CD71, CD34 and HLA-DR) of No and measurement of G-CSF serum leve ls were performed before G-CSF administration (day 0), on the fifth day of G-CSF treatment and days +7 and +30 after the last dose of G-CSF. Results. After G-CSF administration, peripheral blood No presented increase d expression of HLA-DR, CD34, CD14, and CD71, in addition to decreased expr ession of CD10 and CD15 and CD16 fluorescence mean intensity. The phenotypi c changes on No were parallel to serum G-CSF levels. All the phenotypic cha nges returned to baseline values by one month after the end of G-CSF treatm ent. No changes were found in the expression of HLA-DR on T and B-lymphocyt es. Interpretation and Conclusions. These immunophenotypic changes suggest that after G-CSF administration, No from healthy donors who received G-CSF for stem cell mobilization carry transient features of immature phenotype and h ave increased functional activity. (C)1999, Ferrata Storti Foundation.