D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics

Authors
Wiegand, P Lareu, MV Schurenkamp, M Kleiber, M Brinkmann, B
Citation
P. Wiegand et al., D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics, INT J LEGAL, 112(6), 1999, pp. 360-363
Citations number
13
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
ISSN journal
09379827 → ACNP
Volume
112
Issue
6
Year of publication
1999
Pages
360 - 363
Database
ISI
SICI code
0937-9827(199910)112:6<360:DDADTE>2.0.ZU;2-Q
Abstract
Three short tandem repeat (STR) polymorphisms characterized by PCR product length < 175 bp were investigated. D18S535 and D1S1656 contained a 4 bp uni t as basic repeat motif, D10S2325 a 5 bp unit. The heterozygosity rates wer e 0.76 (D18S535), 0.88 (D10S2325) and 0.90 (D1S1656), leading to a combined discrimination power of 0.9999, In contrast to D10S2325 and D18S535, which showed a homogeneous repeat array without any variation in the repeat: mot ifs, repeat length and sequence variation was found for D1S1656. Robust typ ing results could be observed for all three STRs using highly degraded DNA.