Excretion of C-14-Fumonisin B-1, C-14-hydrolyzed fumonisin B-1, and C-14-Fumonisin B-1-fructose in rats

C-14-Fumonisin B-1 (FB1) was produced by Fusarium proliferatum M-5991 in mo dified Myro liquid medium and purified to >95% purity with a specific activ ity of 1.7 mCi/mmol. Nine male and nine female F344/N rats were each dosed by gavage with 0.69 mu mol of C-14-FB1, C-14-hydrolyzed FB1, or C-14-FB1-fr uctose/kg body weight. Urinary excretion of C-14-FB1 and C-14-FB1-fructose was 0.5% and 4.4% of the total dose, respectively, and was similar between male and female rats. Urinary excretion of C-14-hydrolyzed HFB1 was signifi cantly greater (P > 0.05) in female rats as compared with male rats (17.3% vs 12.8% of the total dose, respectively). There were no significant (P > 0 .05) differences in biliary excretion of the three fumonisin compounds with a mean of 1.4% of the dose excreted at 4 h after dosing. Lesser amounts co ntinued to be excreted up to 9.25 h after dosing. Although biliary excretio n of the C-14-FB1, C-14-hydrolyzed FB1, and C-14-FB1-fructose was similar, increased urinary excretion of the C-14-hydrolyzed FB1 as compared to C-14- FB1 and C-14-FB1-fructose indicated a greater absorption of the hydrolyzed form.