I. Pakkala et al., CB1093, A NOVEL VITAMIN-D ANALOG - EFFECTS ON DIFFERENTIATION AND CLONAL GROWTH ON HL-60 AND DE-NOVO LEUKEMIA-CELLS, Leukemia research, 21(4), 1997, pp. 321-326
We studied the effects of a novel vitamin D analog CB1093, EB1089 (one
of the most antileukemic analogs yet) and 1 alpha,25(OH)(2)D-3 both o
n HL-60 cells and cells from 13 AML patients. Differentiation was meas
ured both by induction of superoxide production and non-specific ester
ase. Cell proliferation was assessed by colony assay and H-3-thymidine
incorporation. The effect on serum calcium was measured in rats, The
CB1093 proved to be the most efficient of the analogs tested so far, b
oth in inducing differentiation and in inhibiting proliferation. This,
combined with its low hypercalcemic effect shown here, makes it a pro
mising candidate for preclinical animal studies. (C) 1997 Elsevier Sci
ence Ltd.