CB1093, A NOVEL VITAMIN-D ANALOG - EFFECTS ON DIFFERENTIATION AND CLONAL GROWTH ON HL-60 AND DE-NOVO LEUKEMIA-CELLS

We studied the effects of a novel vitamin D analog CB1093, EB1089 (one of the most antileukemic analogs yet) and 1 alpha,25(OH)(2)D-3 both o n HL-60 cells and cells from 13 AML patients. Differentiation was meas ured both by induction of superoxide production and non-specific ester ase. Cell proliferation was assessed by colony assay and H-3-thymidine incorporation. The effect on serum calcium was measured in rats, The CB1093 proved to be the most efficient of the analogs tested so far, b oth in inducing differentiation and in inhibiting proliferation. This, combined with its low hypercalcemic effect shown here, makes it a pro mising candidate for preclinical animal studies. (C) 1997 Elsevier Sci ence Ltd.