Complexation of 6-deoxy-6-(aminoethyl)amino-beta-cyclodextrin with sodium cholate and sodium deoxycholate

In order to study its guest binding and the inclusion phenomena, 6-deoxy-6- (aminoethyl)amino-beta-cyclodextrin (beta CDN) was synthesised and its bind ing properties examined. The complexation phenomena of sodium cholate (NaC) and sodium deoxycholate (NaDC) with beta CDN has been monitored by the NMR method using C-13 chemical shift data. The method of continuous variation "Job's method" has been used to determine the stoichiometry of these supram olecular complexes. The Job's plot confirms the 1 : 1 supramolecular comple x for NaC: beta CDN and the 1 : 2 supramolecular complex for NaDC: beta CDN . The interaction of NaC and NaDC with beta CDN has been obtained through t wo-dimensional Rotational Nuclear Overhauser Effect Spectroscopy (ROESY) NM R. Equilibrium constants were also obtained from C-13 chemical shift data ( C-1, C-3 & C-4) at different pH values (7, 9, & 11).