MICROPLATE HYBRIDIZATION FOR BORNA-DISEASE VIRUS-RNA IN HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS

Authors
FUJIWARA S TAKAHASHI H NAKAYA T NAKAMURA Y NAKAMURA K IWAHASHI K KAZAMATSURI H IRITANI S KUROKI N IKEDA K IKUTA K
Citation
S. Fujiwara et al., MICROPLATE HYBRIDIZATION FOR BORNA-DISEASE VIRUS-RNA IN HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS, Clinical and diagnostic laboratory immunology, 4(3), 1997, pp. 387-391
Citations number
24
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
Journal title
Clinical and diagnostic laboratory immunology → ACNP
ISSN journal
1071412X
Volume
4
Issue
3
Year of publication
1997
Pages
387 - 391
Database
ISI
SICI code
1071-412X(1997)4:3<387:MHFBVI>2.0.ZU;2-B
Abstract
We developed a simple and sensitive microplate hybridization procedure with which to identify Borna disease virus cDNA in amplified products from human peripheral blood mononuclear cells. The mean values for th e positive PCR products were significant compared with those for any o f the negative products, indicating that this method can be applied to rapidly diagnose a large number of clinical specimens.