Comparison of A68 levels in Alzheimer Diseased and non-Alzheimer's Diseased brain by two ALZ50 based methods

A total of 61 human brain specimens were analyzed with both ELISA and Weste rn Blot using the ALZ50 monoclonal antibody. The brain specimens included: Alzheimer's Disease (AD, n=31), AD/Down's (n=2), Normal (n=14), Parkinson's Disease (n=7), Huntington's chorea (n=2), Wernicke-Korsakov's Encephalopat hy (n=3), and Motor Neuron Disease (n=2). The non-AD cases (n=28) had no de tectable A68 by ELISA, and showed no A68 bands by Western blot, The AD case s (n=33), all were positive for A68 by the ELISA, but only 31 of 33 had vis ible A68 band by Western blot. Additionally, a method for solubilization of A68 is reported.