Exploring biomolecular recognition using optical biosensors

Citation
G. Canziani et al., Exploring biomolecular recognition using optical biosensors, METHODS, 19(2), 1999, pp. 253-269
Citations number
99
Categorie Soggetti
Biochemistry & Biophysics
Journal title
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
ISSN journal
10462023 → ACNP
Volume
19
Issue
2
Year of publication
1999
Pages
253 - 269
Database
ISI
SICI code
1046-2023(199910)19:2<253:EBRUOB>2.0.ZU;2-3
Abstract
Understanding the basic forces that determine molecular recognition helps t o elucidate mechanisms of biological processes and facilitates discovery of innovative biotechnological methods and materials for therapeutics, diagno stics, and separation science. The ability to measure interaction propertie s of biological macromolecules quantitatively across a wide range of affini ty, size, and purity is a growing need of studies aimed at characterizing b iomolecular interactions and the structural elements that drive them. Optic al biosensors have provided an increasingly impactful technology for such b iomolecular interaction analyses. These biosensors record the binding and d issociation of macromolecules in real time by transducing the accumulation of mass of an analyte molecule at the sensor surface coated with ligand mol ecule into an optical signal. Interactions of analytes and ligands can be a nalyzed at a microscale and without the need to label either interactant. S ensors enable the detection of bimolecular interaction as well as multimole cular assembly. Most notably, the method is quantitative and kinetic, enabl ing determination of both steady-state and dynamic parameters of interactio n. This article describes the basic methodology of optical biosensors and p resents several examples of its use to investigate such biomolecular system s as cytokine growth factor-receptor recognition, coagulation factor assemb ly, and virus-cell docking (C) 1999 Academic Press.