Bacterial degradation of hydrocarbons as evidenced by respirometric analysis

The microbial biodegradability of mineral oil and other hydrocarbons, namel y hexane, decane and tetradecane was determined using the Warburg constant volume respirometer. Results of oxygen uptake indicated that hexane and tet radecane were more degradable than mineral oil and decane. Rhodococcus eryt hropolis and Erwinia cancerogena showed the highest (0.866) and lowest (0.1 15) oxygen quotient (Q(O2)) values, respectively, when exposed to mineral o il. Staphylococcus warneri and Enterobacter cloacae showed the highest (2.8 95) and (2.816) Q(O2) values, respectively, when exposed to hexane; whereas E. cloacae and E. cancerogena showed the lowest Q(O2) values (1.289 and 1. 824), respectively. Both R. erythropolis and E. cloacae had the highest Q(O 2) values (2.859 and 2.289), respectively, when exposed to tetradecane. Mor e oxygen was consumed by R. erythropolis than the other bacterial cultures when exposed to all hydrocarbons. In contrast, less oxygen was taken by E, cancerogena than the other bacterial cultures when exposed to all hydrocarb ons, except for hexane.