A sampling of the yeast proteome

In this study, we examined yeast proteins by two-dimensional (2D) gel elect rophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identi fied spots, a good correlation between protein abundance, mRNA abundance, a nd codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to b e insignificant for abundant proteins. Some phosphoproteins were identified . The behavior of proteins in differential centrifugation experiments was e xamined. Such experiments with 2D gels can give a global view of the yeast proteome.