M. Maira et al., Heterodimerization between members of the Nur subfamily of orphan nuclear receptors as a novel mechanism for gene activation, MOL CELL B, 19(11), 1999, pp. 7549-7557
We have recently shown that the orphan nuclear receptor Nur77 (NGFI-B) is m
ost active in transcription when it is interacting with a cognate DNA seque
nce as a homodimer. Further, we have shown that the target for Nur77 dimers
, the Nur response element (NurRE), is responsive to physiological stimuli
in both endocrine and lymphoid cells, whereas other DNA targets of Nur77 ac
tion are not. The Nur77 subfamily also includes two related receptors, Nur-
related factor 1 (Nurr1) and neuron-derived orphan receptor 1 (NOR-1). Ofte
n, more than one member of this subfamily is induced in response to extrace
llular signals. We now show that Nur77 and Nurr1 form heterodimers in vitro
in the presence or absence of NurRE, and we have documented interactions b
etween these proteins in vivo by using a two-hybrid system in mammalian cel
ls. These heterodimers synergistically enhance transcription from NurRE rep
orters in comparison to that seen with homodimers. The naturally occurring
NurRE from the pro-opiomelanocortin gene preferentially binds and activates
transcription in the presence of Nur77 homo- or heterodimers, while a cons
ensus NurRE sequence does not show this preference. Taken together, the dat
a indicate that members of the Nur77 subfamily are most potent as heterodim
ers and that different dimers exhibit target sequence preference. Thus, we
propose that a combinatorial code relying on specific NurRE sequences might
be responsible for the activation of subsets of target genes by one of the
members of the Nur77 subfamily of transcription factors.