K. Harada et al., PROTEOLYSIS OF ANKYRIN AND NA+ K+-ATPASE IN POSTMORTEM RAT-BRAIN - ISCALPAIN INVOLVED/, Forensic science international, 86(1-2), 1997, pp. 77-85
Ankyrin links the fodrin-based cytoskeleton to membrane proteins such
as Na+/K+-ATPase, thereby maintaining cellular integrity. Immunoblotti
ng by antibody raised against erythrocyte ankyrin demonstrated the pro
teolysis of ankyrin, which was highly correlated with postmortem inter
val (0-24 h). Proteolysis in the postmortem brain generated the 160-kD
a fragment with an identical size as the fragment formed after in vitr
o proteolysis by calpain. Although mu M Ca2+ induced the proteolysis i
n the homogenate, the presence of mu-calpain was not demonstrated by i
mmunoblotting using the antibody that reacts with large subunits both
of mu- and m-calpains. Na+/K+-ATPase was also proteolyzed in the postm
ortem brain. (C) 1997 Elsevier Science Ireland Ltd.