Separation of enantiomers of some 1,4-piperazine derivatives of aryloxyaminopropanols on a vancomycin chiral stationary phase

The behaviour of a vancomycin chiral stationary phase (Chirobiotic V) towar ds changes in organic and ionic modifiers in the mobile phase was investiga ted in order to deduce suitable conditions for the liquid chromatographic e nantioseparation of a series of 1,4-piperazine derivatives of aryloxyaminop ropanols. Acetonitrile and methanol as non-ionic modifiers were tested in t he mobile phase while different aliphatic carboxylic acids (formic acid, pr opionic acid, hexanoic acid, oxalic acid, succinic acid) and bases (triethy lamine, trimethylamine, ammonia) were used as ionic modifiers. The influenc e of the nature and concentration of these modifiers on retention, selectiv ity and resolution was investigated.