The complete genomic DNA sequence of the aero/anaero-facultative archaebact
erium, Thermoplasma volcanium GSS1, has been determined. A number of DNA fr
agments were cloned by using the lambda, cosmid, and BAC systems, and seque
nced. The remaining 30 gaps were bridged by DNA fragments constructed using
the polymerase chain reaction. The repetition in sequencing the same base
positions was 13.1+/-7.5 fold. The alignment of the DNA fragments and the c
ompleteness of the genomic sequence were confirmed by the consistency of th
e genomic sequence with the lengths and partial sequences of a second set o
f DNA fragments that altogether covered 88% of the genome. The number of ba
ses found in the genomic sequence is 1,584,799, with a G/C content of 39.9%
. The combination of the four types of bases in the new genomic sequence is
compared with those in known genomic sequences of similar sizes.