Carbohydrate-derivatized self-assembled monolayers (SAMs) are used as a mod
el system to address issues involving cell-surface carbohydrate-protein int
eractions. Here we examine the influence of carbohydrate surface density on
protein-binding avidity. We show that the binding selectivity of Bauhinia
purpurea lectin switches from one carbohydrate ligand to another as the sur
face density of the carbohydrate ligands increases from values of chi(sugar
) approximate to 0.1-1.0. Polyvalent binding is possible at all surface den
sities investigated; hence, the switch in selectivity is not due simply to
the achievement of a critical density that permits polyvalent contacts. Ins
tead, secondary interactions at high surface densities promote a switch in
carbohydrate-binding selectivity. These findings may have implications for
how changes in the composition and the density of cell-surface carbohydrate
s influence biological recognition processes and regulatory pathways.