Accumulation of specific RNAs encoding transcriptional factors and stress response proteins against a background of severe depletion of cellular RNAsin cells infected with herpes simplex virus 1
Nn. Khodarev et al., Accumulation of specific RNAs encoding transcriptional factors and stress response proteins against a background of severe depletion of cellular RNAsin cells infected with herpes simplex virus 1, P NAS US, 96(21), 1999, pp. 12062-12067
Citations number
45
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Herpes simplex virus 1 encodes several functions to preclude the shutoff of
host response to infection, including degradation of mRNA immediately afte
r infection. To determine whether any cellular mRNAs accumulate in infected
cells against a background of severe loss of host RNA, we hybridized cDNAs
derived from three different cell lines infected with wild type and a muta
nt virus to a DNA array containing probes for 588 human genes representing
different functional groups. The results were that (i) infected cells accum
ulated at levels above those of mock-infected cells, a small number of tran
scripts representing transcriptional factors that could regulate gene expre
ssion both positively and negatively, and one stress response protein (GADD
45), (ii) the amount and nature of the accumulated transcripts showed limit
ed variability depending on the cell and virus, and (iii) at least some of
the proteins encoded by the accumulated transcripts could benefit either th
e virus or the host.