Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis

Citation
J. Sikorski et al., Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis, SYST APPL M, 22(3), 1999, pp. 393-402
Citations number
43
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
07232020 → ACNP
Volume
22
Issue
3
Year of publication
1999
Pages
393 - 402
Database
ISI
SICI code
0723-2020(199909)22:3<393:AOGDAR>2.0.ZU;2-S
Abstract
Molecular fingerprinting procedures including random amplified polymorphic DNA-PCR (RAPD), repetitive extragenic palindromic PCR (rep-PCR) with REP, E RIC, and BOX primers and multilocus enzyme electrophoresis (MLEE) were used for genotypic characterization of 16 P. stutzeri strains originally isolat ed from marine, waste water, clinical and soil samples. A distinct genotype of each strain and overall great genotypic diversity were found within P. stutzeri. Cluster analysis (UPGMA) of the electrophoretic patterns of all P CR-based methods used resulted in concordant grouping of 8 strains. With th e other strains conflicting clustering was noticed. The variability of clus tering in PCR-based analyses suggested the occurrence of chromosomal rearra ngements. When RAPD-, rep-PCR and MLEE fingerprints were used in a cluster analysis of combined electrophoretic patterns, the P. stutzeri strains coul d be differentiated into seven distinct genotypic groups. These results sup ported the subdivision of the species in several genomovars and reproduced, with higher resolution, the strain grouping after 16 rRNA phylogenetic rec onstruction. The combined use of several fingerprint-based genotypic analys es results in higher resolutive strain clustering by UPGMA than each of the single ones analyzed separately. Additionally, this combination of individ ual typings proved to be reliable of the determination of the great genotyp ic diversity and relationships among the P. stutzeri strains.