Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis
J. Sikorski et al., Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis, SYST APPL M, 22(3), 1999, pp. 393-402
Molecular fingerprinting procedures including random amplified polymorphic
DNA-PCR (RAPD), repetitive extragenic palindromic PCR (rep-PCR) with REP, E
RIC, and BOX primers and multilocus enzyme electrophoresis (MLEE) were used
for genotypic characterization of 16 P. stutzeri strains originally isolat
ed from marine, waste water, clinical and soil samples. A distinct genotype
of each strain and overall great genotypic diversity were found within P.
stutzeri. Cluster analysis (UPGMA) of the electrophoretic patterns of all P
CR-based methods used resulted in concordant grouping of 8 strains. With th
e other strains conflicting clustering was noticed. The variability of clus
tering in PCR-based analyses suggested the occurrence of chromosomal rearra
ngements. When RAPD-, rep-PCR and MLEE fingerprints were used in a cluster
analysis of combined electrophoretic patterns, the P. stutzeri strains coul
d be differentiated into seven distinct genotypic groups. These results sup
ported the subdivision of the species in several genomovars and reproduced,
with higher resolution, the strain grouping after 16 rRNA phylogenetic rec
onstruction. The combined use of several fingerprint-based genotypic analys
es results in higher resolutive strain clustering by UPGMA than each of the
single ones analyzed separately. Additionally, this combination of individ
ual typings proved to be reliable of the determination of the great genotyp
ic diversity and relationships among the P. stutzeri strains.