Determination of glucose and fructose in mixtures by a kinetic method withchemiluminescence detection

A kinetic method of analysis is described, based on oxidation by periodate for the determination of glucose and fructose in sample solutions. The meth od combines flow injection and chemiluminescence (CL) detection. The detect ion is based on the CL signal generated during oxidation of pyrogallol by p eriodate. The method is selective for the determination of vic-diols and po lyols; the method is designed to determine hexose contents as low as 200 mu g. However, the method can be tailored for even lower amounts (10 times le ss). When the total hexose concentration is known, the individual fructose and glucose content can be determined from the rate constant that best-fits the kinetic data; the relative standard deviation of three independent kin etic experiments with the same ratio of concentrations was found to be with in 2% and the standard error of the rate constant from the fitting treatmen t was found to be less than 10% in all samples of different concentration r atios between glucose and fructose. The method can be automated and control led by computer software. Data points from two kinetic experiments with dif ferent sample quantities in the reaction mixture are required for the deter mination of the total hexose content and the individual fractions of glucos e and fructose. (C) 1999 Elsevier Science B.V. All rights reserved.