A chemiluminescence fiber-optic biosensor for detection of DNA hybridization

Authors
Zhang, GJ Zhou, YK Yuan, JW Ren, S
Citation
Gj. Zhang et al., A chemiluminescence fiber-optic biosensor for detection of DNA hybridization, ANAL LETTER, 32(14), 1999, pp. 2725-2736
Citations number
19
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL LETTERS
ISSN journal
00032719 → ACNP
Volume
32
Issue
14
Year of publication
1999
Pages
2725 - 2736
Database
ISI
SICI code
0003-2719(1999)32:14<2725:ACFBFD>2.0.ZU;2-S
Abstract
A novel fiber-optic DNA biosensor based on chemiluminescence is proposed. D NA probes were covalently immobilized onto the distal end of the optical fi ber bundle. Hybridization of HRP (horseradish peroxidase)-labeled complemen tary nucleotides to the immobilized probes was detected by enhanced chemilu minescence. The detection limit (S/N=3) is 0.1 ppm. The approach is shown t o be specific, sensitive and capable of diagnostic applications.