'Environmental enrichment' is often considered to improve captive animal we
lfare. However, some studies using male mice, Mus musculus, indicate that i
ncreasing cage complexity, increases aggression. Limited evidence suggests
that enrichment differs in ifs effects on behaviour and physiology between
strains; but behaviour also differs between strains in non-enriched environ
ments. Differ ences in enrichment type, evaluation methods, and strains use
d, have caused difficulty in interpreting the efficacy of environmental enr
ichment in improving welfare. Using enrichment suitable for commercial labo
ratories (nesting material and a Perspex tunnel), we physiological response
s among males of six strains housed in iron-enriched standard polypropylene
cages with those housed in 'enriched' cages. Outbred ICR(CD-1) and TO mice
, and inbred BALB/c mice were more aggressive than C57BL/6, CBA/Ca and DBA/
2 mice, which exhibited low levels of aggression typical of most inbred str
ains. Enrichment did not significantly affect aggression levels. Animals in
enriched cages spent more time investigating the internal cage environment
, eating and drinking, and in stereotypic behaviour patterns, although leve
ls differed between strains. The greatest increase in stereotypy levels (ba
r-related stereotypies,pies) with enrichment was found in DBA/2 mice. Highe
r testosterone levels were maintained over the study, period ill mice house
d in enriched cages, and in more aggressive strains. IgG levels were also h
igher in mice housed in enriched cages, and in the outbred strains ICR(CD-1
) and TO compared with inbred strains. The relationship between aggression,
testosterone and 'enrichment' suggests, that increasing complexity in labo
ratory cages may increase a naturally, selected territorial response in som
e strains. The implications for strain-specific welfare are discussed.