EFFECT OF HUMAN RENAL CALCULI EXTRACT ON IN-VITRO CALCIUM-OXALATE MONOHYDRATE CRYSTALLIZATION

Human renal calculi surgically removed from kidney stone patients were obtained and chemically analysed. Stones with calcium oxalate (CaOx) as the major component were collected, pulverised to a fine powder and then demineralised at 4 degrees C for 2-7 days in 0.2M EDTA (pH 7.5). The protein contents of extracts collected at different time interval s were determined. Fixed amounts of calcium oxalate monohydrate (COM) crystals (prepared by dropwise mixing equal volumes of 0.01 M CaCl2 an d 0.01 M NaOx) were incubated in a metastable solution containing C-14 -oxalate and known amounts of EDTA extracts. The studies revealed that the human renal calculi extract contains biomolecules that cannot onl y inhibit but also promote the growth of preformed COM crystals.