Proteolytic activity of the adult worm extracts of Onchocerca volvulus.

Studying proteolytic activity of Onchocerca volvulus (nematode causing "riv er blindness") shows that it is able to digest a variety of subtrates such as: azoalbumine, azocoll and elastin-orcein with specific activity of 0.28, 0.57 and 1.48 mg/hour/mg of extract respectively These enzymes are active at various pH such as pH 5.0 8.0 and 10.0 with highest activity at pH 8.0. The effect of specific inhibitors and activators indicates that the extract might contain serine, metallo and thioproteases. The electrophoresis of the extract on a polyacrylamide gel copolymerised wi th gelatine shows many proteins with enzymatic activities with molecular we ight of 16.6 43.6 45.7, 56.2, 60.2, 61.6 and 63.1 KD respectively. The Onchocerca volvulus worm contains proteases of various enzymatic activi ties: a non specific activity on protein such as on azoalbumin and specific activities on collagen and elastin. These enzymes could play an important role in the survival of parasites in human hosts.