Identification of IgG-specific oligoclonal banding in serum and cerebrospinal fluid by isoelectric focusing: Description of a simplified method for the diagnosis of neurological disorders

Clinical laboratory procedures using cerebrospinal fluid (CSF) to assist in the diagnosis of multiple sclerosis (MS) are based essentially on the dete rmination of oligoclonal bands (OB) in CSF and not in serum. To date, the t echniques using isoelectric focusing (IEF) have represented one of the most efficient methods for detecting supernumerary bands in CSF but unfortunate ly were not always compatible with a routine use. Here, we describe a revis ed method of IEF suitable for the diagnosis of neurological disorders. The kit-based technique was simplified and the present procedure allowed an eas ier execution, reduced the overall analysis time and permitted an uncomplic ated intepretation of oligoclonal profiles. Moreover, the technique develop ed was very sensitive and specific for the diagnosis of MS diagnosis. In co nclusion, the IEF protocol described in this report is suitable for OB dete ction in any laboratory.