Thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (Anoplopoma fimbria), black rockfish (Sebastes melanops), and chub mackerel (Scomber japonicus)
Bd. Johnston et al., Thermal modulation of the toxicokinetics of benzo[a]pyrene in isolated hepatocytes of sablefish (Anoplopoma fimbria), black rockfish (Sebastes melanops), and chub mackerel (Scomber japonicus), COMP BIOC C, 124(2), 1999, pp. 157-164
Citations number
23
Categorie Soggetti
Pharmacology & Toxicology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY
Hepatocytes from sablefish (Anoplapoma fimbria), black rockfish (Sebastes m
elanops) and chub mackerel (Scomber japonicus) were isolated from 11 degree
s C acclimated animals. The uptake, metabolism, and excretion of benzo[a]py
rene (B[a]P) in hepatocytes was measured at 6, 11 and 19 degrees C. Chub ma
ckerel hepatocyte uptake rates were significantly lower (0.012 +/- 0.003 mu
g/s per g cell) at 11 degrees C than black rockfish (0.028 +/- 0.009 mu g/
s per g cell) or sablefish (0.032 +/- 0.012 mu g/s per g cell) hepatocytes
at all temperatures. Hepatocytes metabolized B[a]P to phase I (1-8%) and ph
ase II (92-99%) metabolites. Accumulation of phase II metabolites was lower
in chub mackerel hepatocytes (0.016 +/- 0.004 mu g/h per g cell), than bla
ck rockfish (0.052 +/- 0.012 mu g/h per g cell), or sablefish hepatocytes (
0.060 +/- 0.015 mu g/h per g cell). Phase II metabolite accumulation increa
sed greatest with temperature in chub mackerel hepatocytes (Q(10) = 1.94 +/
- 0.30), followed by sablefish (Q(10) = 1.65 +/- 0.30), and rockfish (Q(10)
= 1.38 +/- 0.30). Sablefish hepatocytes had higher excretion rates of phas
e II metabolites (0.010 +/- 0.0023 mu g/h per g cell), than mackerel (0.004
6 +/- 0.0009 mu g/h per g cell) or rockfish hepatocytes (0.0029 +/- 0.0008
mu g/h per g cell). Phase II metabolite excretion rates increased with temp
erature only in sablefish hepatocytes (Q(10) = 1.67 +/- 0.76). These differ
ences in toxicokinetics may indicate distinct consequences for various spec
ies exposed to xenobiotics. (C) 1999 Elsevier Science Inc. All rights reser
ved.