A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1)

Authors
Gaisne, M Becam, AM Verdiere, J Herbert, CJ
Citation
M. Gaisne et al., A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1), CURR GENET, 36(4), 1999, pp. 195-200
Citations number
29
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CURRENT GENETICS
ISSN journal
01728083 → ACNP
Volume
36
Issue
4
Year of publication
1999
Pages
195 - 200
Database
ISI
SICI code
0172-8083(199910)36:4<195:A'MISC>2.0.ZU;2-R
Abstract
The HAP1 gene encodes a complex transcriptional regulator of many genes inv olved in electron-transfer reactions and is essential in anaerobic or heme- depleted conditions. We show here that strains derived from S288c carry a d efective Ty1 element inserted in the 3' region of the HAP1 ORF. This mutant allele acts as a HAP1 null allele in terms of cytochrome c expression and CYC1 UAS1-dependent transcription, but is able to sustain limited growth in heme-depleted conditions.