Mm. Moline et al., Directionality of Wingless protein transport influences epidermal patterning in the Drosophila embryo, DEVELOPMENT, 126(19), 1999, pp. 4375-4384
Active endocytotic processes are required for the normal distribution of Wi
ngless (Wg) protein across the epidermal cells of each embryonic segment. T
o assess the functional consequences of this broad Wg distribution, we have
devised a means of perturbing endocytosis in spatially restricted domains
within the embryo. We have constructed a transgene expressing a dominant ne
gative form of shibire (shi), the fly dynamin homologue. When this transgen
e is expressed using the GALA-UAS system, we find that Wg protein distribut
ion within the domain of transgene expression is limited and that Wg-depend
ent epidermal patterning events surrounding the domain of expression are di
srupted in a directional fashion. Our results indicate that Wg transport in
an anterior direction generates the normal expanse of naked cuticle within
the segment and that movement of Wg in a posterior direction specifies div
erse denticle cell fates in the anterior portion of the adjacent segment. F
urthermore, we have discovered that interfering with posterior movement of
Wg rescues the excessive naked cuticle specification observed in naked (nkd
) mutant embryos. We propose that the nkd segment polarity phenotype result
s from unregulated posterior transport of Wg protein and therefore that wil
d-type Nkd function may contribute to the control of Wg movement within the
epidermal cells of the segment.