Application of temperature and applied voltage programming in micellar electrokinetic capillary chromatography

Temperature and voltage programming modes were utilized to optimize selecti vity and increase the eluting rate of strongly retained compounds in micell ar electrokinetic chromatography, Separations obtained by applying temperat ure, voltage, and a simultaneous combination of temperature and voltage gra dient in micellar electrokinetic capillary chromatography were compared wit h separations performed under isothermal and constant voltage conditions. A complete separation of all the constituents of the test mixture was only a chieved in the temperature programming run and in a combination of temperat ure and voltage programming modes, Simultaneous variations of column temper ature and applied voltage during a separation run, yielded a 30% reduction in the total analysis time when compared to a temperature gradient alone. T emperature programming and voltage programming modes may be the gradient me thods of choice because of the considerable technical difficulties involved in performing linear solvent gradient elution in micellar electrokinetic c hromatography.