Prototypical test substances for a reversed phase in a retention parametermodel

A set of four prototypical substances (naphthalene, acetanilide, phenol, an d benzonitrile) is selected from 26 different analytes used by Seibert and Poole for characterization of the retention behavior of 20 liquid chromatog raphic systems of a C18 reversed phase with methanol/water and acetonitrile /water as mobile phases. Retention data of 22 different analytes can be cal culated from the retention data of the set of prototypical substances with a mean standard deviation of 5.4%. Two phase system maps similar to that su ggested by Poole et al. are provided for an octadecylsiloxane-bonded silica sorbent with methanol and acetonitrile as cosolvents with water.