Production of specific antibody and T helper 1-dominant cytokine elicited by dendritic cells genetically modified with an adenovirus vector

In this study we examined how dendritic cells (DCs) transduced with an aden ovirus Vector encoding a model tumor antigen (beta-galactosidase; beta-gal) would influence the humoral immune response to this antigen. Mice immunize d with LacZ transduced DCs by an adenovirus vector could produce more anti- beta-gal antibody, especially of IgG2a subclass, than mice immunized with D Cs alone, although the amount of serum IgG antibody did not increase. Compa red with mice immunized with DCs alone, splenocytes of mice immunized with LacZ transduced DCs could produce more interferon-gamma (IFN-gamma) against the beta-gal derived, H-2L(d)-restricted nonapeptide (TPHPARIGL) in a dose -dependent manner. These data suggest that DCs transduced with an adenoviru s vector encoding the model tumor antigen could induce the T helper I (Th1) dominant response against the model tumor antigen. (C) 1999 Elsevier Scien ce B.V. All rights reserved.