A. Siripinyanond et Rm. Barnes, Flow field-flow fractionation-inductively coupled plasma mass spectrometryand metal speciation in proteins: A feasibility study, J ANAL ATOM, 14(9), 1999, pp. 1527-1531
The feasibility of trace metal speciation in proteins directly using flow f
ield-flow fractionation-inductively coupled plasma mass spectrometry (FlFFF
-ICP-MS) was investigated. The effect on the separation of the FlFFF system
cross flow rate was demonstrated for a carbonic anhydrase and alcohol dehy
drogenase mixture. Preliminary studies with FlFFF-ICP-MS were applied to se
veral protein standards including metallothionein, carbonic anhydrase, ceru
loplasmin, alcohol dehydrogenase, and thyroglobulin. Cadmium was detected i
n metallothionein; copper and zinc in carbonic anhydrase, alcohol dehydroge
nase, and ceruloplasmin, and iodide in thyroglobulin.