In-vitro antifungal susceptibilities of Basidiobolus and Conidiobolus spp.strains

The in-vitro antifungal susceptibilities of nine isolates belonging to Basi diobolus spp. and seven to Conidiobolus spp. against six antifungals (ampho tericin B, ketoconazole, miconazole, itraconazole, fluconazole and flucytos ine) were tested. A broth microdilution method, generally following the NCC LS guidelines, was used. Inoculum concentrations of the order of 100 cfu/mL were obtained by culturing fungi in a broth medium (Czapeck broth suppleme nted with 2% Tween 80 and 0.07% agar). MICs and MFCs were highly variable a nd isolate-dependent, with the exception of those of flucytosine which were constantly very high. In general, however, Basidiobolus spp. displayed low MICs of fluconazole, itraconazole, ketoconazole and miconazole, and Conidi obolus spp. were resistant to all antifungals tested.