Hj. Zhong et al., SEPARATION AND QUANTIFICATION OF PECTINS USING CAPILLARY ELECTROPHORESIS - A PRELIMINARY-STUDY, Carbohydrate polymers, 32(1), 1997, pp. 27-32
A rapid method for the analysis of the degree of esterification (DE) o
f pectin is presented. The migration time of pectin in capillary elect
rophoresis (CE) is a function of the DE, and separation of pectins wit
h different DE values has been demonstrated. Using the average charge
per residue, z = -(100-DE)/100, a linear relationship is found between
pectin charge and mobility. For a fixed charge mobility increases wit
h decreasing ionic strength, but does not depend significantly on whet
her the pectin charge distribution is blockwise or random. This CE met
hod can be used for the quantitative detection of aqueous solutions of
pectins in the concentration range 0.5-5 mg ml(-1) using a 50 mM, pH
7.0 phosphate background electrolyte and u.v. detection at 192 nm. (C)
1997 Elsevier Science Ltd.