Root exudates from Tephrosia plants contained a rock-iron [Fe(OH)(3)]-solub
ilizing compound. This was purified to apparent homogeneity and appears to
possess an iron-binding phenolic moiety linked to a proteinaceous component
that may act as a receptor. The lyophilized purified fraction showed maxim
um Fe(OH)(3)-solubilizing activity at 50 degrees C under acidic pH. Its act
ivity was influenced by trace ions (Co, Cu, Zn, B, Mo, Mn) and toxic metal
ions (Ni, Al, Pb, Cr, Hg), but ions such as Na, K, Mg, and Ca had no signif
icant effects on its Fe(OH)(3)-solubilizing activity.