GEOGRAPHIC-VARIATION OF THE PREDICTIVE VALUES OF GENOMIC MUTATIONS ASSOCIATED WITH STREPTOMYCIN RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS

Specific mutations associated with resistance to streptomycin (SM) in Mycobacterium tuberculosis suggest themselves for its rapid prediction . However, as with any diagnostic test, their predictive values are de pendent on their prevalences. In this report, SM resistance associated mutations in the rrs and rpsL genes of 25 SM resistant isolates from Germany and 25 SM resistant isolates from Sierra Leone were characteri zed and compared. Mutations in the rrs gene were infrequent in isolate s from both localities (20% and 12%, respectively) and thus of limited predictive values. In contrast, rpsL mutations were found in 48% of t he German isolates but only in 24% of the isolates from Sierra Leone. It is concluded that the predictive values of mutations in this gene m ay vary significantly with the origin of the samples under investigati on. (C) 1997 Academic Press Limited.