Brca1 controls homology-directed DNA repair

Citation
Me. Moynahan et al., Brca1 controls homology-directed DNA repair, MOL CELL, 4(4), 1999, pp. 511-518
Citations number
49
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR CELL
ISSN journal
10972765 → ACNP
Volume
4
Issue
4
Year of publication
1999
Pages
511 - 518
Database
ISI
SICI code
1097-2765(199910)4:4<511:BCHDR>2.0.ZU;2-R
Abstract
Germline mutations in BRCA1 confer a high risk of breast and ovarian tumors . The role of BRCA1 in tumor suppression is not yet understood, but both tr anscription and repair functions have been ascribed. Evidence that BRCA1 is involved in DNA repair stems from its association with RAD51, a homolog of the yeast protein involved in the repair of DNA double-strand breaks (DSBs ) by homologous recombination. We report here that Brca1-deficient mouse em bryonic stem cells have impaired repair of chromosomal DSBs by homologous r ecombination. The relative frequencies of homologous and nonhomologous DNA integration and DSB repair were also altered. The results demonstrate a car etaker role for BRCA1 in preserving genomic integrity by promoting homologo us recombination and limiting mutagenic nonhomologous repair processes.