In situ hybridization - Theory and practice

In situ hybridization is a technique to determine and localize target nucle ic acids in morphologically preserved tissue sections. Recent advances in m ethods have greatly increased the sensitivity of the technique, and it is c urrently possible to detect extremely few copies of any given target sequen ce with nonisotopic methods In this teaching review, we integrate theoretic al background, technical considerations, and guidelines for usage for this important component of molecular diagnosis.