Rmd. Lastrucci et al., Development of a simple multiplex polymerase chain reaction for the simultaneous detection of factor V Leiden and prothrombin 20210A mutations, MOL DIAGN, 4(3), 1999, pp. 247-250
Background: The demand for thrombophilia testing at the molecular level is
increasing, and consequently, the work load of the routine molecular labora
tory is also increasing. Efforts to lighten the work load, economize on tim
e, and strive for reduced costs while still maintaining quality assurance,
are thus necessary.
Methods and Results:A multiplex polymerase chain reaction (PCR) for the det
ection of factor V Leiden and prothrombin 20210A mutations was designed tha
t enables the use of the same inexpensive restriction enzyme, controls for
the digestion, and produces easily interpretable results.
Conclusion: The use of this new multiplex PCR and digestion analysis enable
d us to simultaneously perform a routine screen for factor V Leiden and pro
thrombin 20210A mutations.