Introduction of human chromosome 13 into retinoblastoma-negative metastatic human prostate cancer cells increases their sensitivity to growth inhibition by transforming growth factor-beta 1
Ms. Steiner et Ct. Anthony, Introduction of human chromosome 13 into retinoblastoma-negative metastatic human prostate cancer cells increases their sensitivity to growth inhibition by transforming growth factor-beta 1, MOL UROL, 3(3), 1999, pp. 153-161
Like many other carcinomas, prostate cancer develops resistance to inhibiti
on by transforming growth factor (TGF)-beta 1 during oncogenesis, One propo
sed mechanism of TGF-beta 1 action posits action of the retinoblastoma prot
ein (pRb) to suppress c-myc transcription to inhibit cellular proliferation
. A metastatic human prostate cancer cell line, DU145, has both nonfunction
al pRb and markedly reduced sensitivity to TGF-beta 1 growth inhibition. Th
e defective rb gene in DU145 cells was replaced by a normal rb allele by mi
crocell fusion of chromosome 13, Two subclones, DU145-Cl-I and DU145-Cl-II,
were studied in vitro to determine whether the pRb restoration increased s
ensitivity to the inhibitory effects of TGF-beta 1, By reverse transcriptas
e-polymerase chain reaction, increased sensitivity to the inhibitory effect
s of TGF-beta 1, By reverse transcriptase-polymerase chain reaction, parent
al DU145 cells had TGF-beta receptors of Type I and Type II. Introduction o
f chromosome 13 reduced the growth rate and prolonged the G(1) phase compar
ed with the parental DU145 cell line. Moreover, responsiveness to TGF-beta
1 growth inhibition was restored in a dose-dependent manner. Transcription
of c-myc was not altered by TGF-beta 1 growth inhibition. Thus, DU145 cells
presumably required the presence of wildtype rb to become growth inhibited
that is independent of c-myc transcription. As the entire chromosome 13 wa
s introduced, unknown tumor suppressor genes, not only rb, may be responsib
le for the restoration of TGF-beta 1 growth inhibition.