Deletogenic activity of 1,2 : 7,8-diepoxyoctane in the Salmonella typhimurium tester strain TA102

1,2:7,8-Diepoxyoctane (DEO), whose deletogenic activity was first demonstra ted in ad-3 system of Neurospora crassa and then in different species, has been tested in Salmonella typhimurium tester strain TA102 (hisG428(Ochre)). It was confirmed that it is a direct acting mutagen and was found that its activity is stimulated with the S9 mix. Obtained His(+) revertants were sc reened on their response to the histidine analog, N-(2-thiazolyl)-DL-alanin e (ThiAla). Thirty-two percent of spontaneous and 52% of DEO-induced revert ants were resistant to the analog while no resistance was observed among th ose induced with 4-nitroquinoline-N-oxide (4NQO). Resistance to ThiAla was interpreted as due to small deletions surrounding the target TAA codon in h isG428(Ochre). Thus, at least two simple test-systems, ad-3 of N. crassa an d hisG428(Ochre) of S. typhimurium, gave compatible results and might be us eful in searching of deletogens. (C) 1999 Elsevier Science B.V. All rights reserved.