Ml. Fung et al., Sodium homeostasis in rat hippocampal slices during oxygen and glucose deprivation: role of voltage-sensitive sodium channels, NEUROSCI L, 275(1), 1999, pp. 41-44
The breakdown in brain ionic homeostasis during energy deprivation (anoxic
depolarization [AD]) is intimately linked to neuronal injury. We studied th
e role of one particular route of Na+ influx, voltage-sensitive Na+ channel
s, in the AD induced by O-2 and/or glucose deprivation. We recorded extrace
llular Na+ concentration ([Na+](e)) and direct current potential (DCP) in t
he CA1 stratum pyramidale of hippocampal slices using Na+-selective microel
ectrodes. Tetrodotoxin (0.1-1 mu M) delayed the occurrence of AD and reduce
d the peak change in both [Na+](e) and DCP during AD. However the tetrodoto
xin effects were overcome by a concomitant reduction in extracellular gluco
se during anoxia. We conclude that: (1) the activation of voltage-gated Na channels is involved in the triggering of AD; (2) there may be a critical
level of energy depletion when AD occurs and different mechanisms may under
lie AD during hypoxia, compared to O-2 and glucose deprivation. (C) 1999 El
sevier Science Ireland Ltd. All rights reserved.