Apoptosis induction in nonirradiated human HL-60 and murine NSO/2 tumor cells by photoproducts of indole-3-acetic acid and riboflavin

Authors
Edwards, AM Barredo, F Silva, E De Ioannes, AE Becker, MI
Citation
Am. Edwards et al., Apoptosis induction in nonirradiated human HL-60 and murine NSO/2 tumor cells by photoproducts of indole-3-acetic acid and riboflavin, PHOTOCHEM P, 70(4), 1999, pp. 645-649
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PHOTOCHEMISTRY AND PHOTOBIOLOGY
ISSN journal
00318655 → ACNP
Volume
70
Issue
4
Year of publication
1999
Pages
645 - 649
Database
ISI
SICI code
0031-8655(199910)70:4<645:AIINHH>2.0.ZU;2-A
Abstract
The effect of the photoproducts of indole-3-acetic acid sensitized by ribof lavin on nonirradiated human HL-60 and murine NSO/2 tumor cells was studied . Severe damage with a dose-response effect was observed on both cell types . The effect was greater than that previously described for the tryptophan riboflavin photoproducts. Electron microscopy studies and flow cytometry an alysis of DNA fragmentation allowed us to conclude that the photoproducts s tudied in this work induce cell death by an apoptotic mechanism.