Postsynaptic clustering of gamma-aminobutyric acid type A receptors by thegamma 3 subunit in vivo

Synaptic localization of gamma-aminobutyric acid type A (GABAA) receptors i s a prerequisite for synaptic inhibitory function, but the mechanism by whi ch different receptor subtypes are localized to postsynaptic sites is poorl y understood. The gamma 2 subunit and the postsynaptic clustering protein g ephyrin are required for synaptic localization and function of major GABA(A ) receptor subtypes. We now show that transgenic overexpression of the gamm a 3 subunit in yt subunit-deficient mice restores benzodiazepine binding si tes, benzodiazepine-modulated whole cell currents, and postsynaptic miniatu re currents, suggesting the formation of functional, postsynaptic receptors . Moreover, the gamma 3 subunit can substitute for gamma 2 in the formation of GABA(A) receptors that are synaptically clustered and colocalized with gephyrin in vivo. These clusters were formed even in brain regions devoid o f endogenous gamma 3 subunit indicating that the factors present for cluste ring of gamma 2 subunit-containing receptors are sufficient to cluster gamm a 3 subunit-containing receptors. The GABA(A) receptor and gephyrin-cluster ing properties of the ectopic gamma 3 subunit were also observed for the en dogenous gamma 3 subunit but only in the absence of the gamma 2 subunit, su ggesting that the gamma 3 subunit is at a competitive disadvantage with the gamma 2 subunit for clustering of postsynaptic GABA(A) receptors in wild-t ype mice.