A study of using 2,4,6-trihydroxyacetophenone as matrix in matrix - assisted laser desorption/ionization mass spectrometry for DNA detection

This paper reports the study on matrix - assisted laser desorption/ionizati on time - of - flight mass spectrometry( MALDI - TOF - MS) of DNA, using 2, 4, 6 - trihydroxyacetophenone (2, 4, 6 - THAP) as matrix. The results show that 2,4,6 - THAP has high efficiency in desorbing and ionizing DNA molecu les: for [M - H](-) of d(T)(10), the resolution is 1130.0,the signal - to - noise ratio is 366.0, and the detection limit is 5 x 10-14mol; it can meas ure mix - based DNA with molecular weight higher than 14800. In order to im prove desorption/ionization effects,the purification methods for DNA sample s are established.