Highly efficient molecule imprinting chiral stationary phase utilizing combined basic functional monomers

Molecular imprinting polymers (MIP) against amino acid derivatives utilizin g amide plus 2-vinylpyridine as combined basic functional monomers were pre pared and evaluated as chiral stationary phase for high - performance liqui d chromatography. The MIPs prepared at optimized ratio of the imprinting mo lecule to combined functional monomers show excellent chiral separation abi lity to the imprinting enantiomer and its structurally related enantiomers. The effect of chemical group of the analyse on the chiral separation was i nvestigated. Hydrogen bonding and strong ionic interaction between the func tional monomer and the analyse were found.