Antibody (Ab) labeled with alkaline phosphatase was separated and detected
by a capillary electrophoresis system equipped with a laser induced fluores
cence detection system. The combination of B/F separation and the LIF detec
tion of the label enzyme is potentially useful for a highly sensitive detec
tion of antigen (Ag). After B/F separation by capillary electrophoresis (CE
), label enzymes catalyze the hydrolysis of fluorescein diphosphate in the
running buffer for several minutes, producing fluorescein. Experiments with
various ratios of Ab to Ag revealed that the faster peak was the complex o
f Ab and Ag, and the other was free Ab. (C) 1999 Elsevier Science B.V. All
rights reserved.