Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae

Authors
Kontoyiannis, DP Sagar, N Hirschi, KD
Citation
Dp. Kontoyiannis et al., Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae, ANTIM AG CH, 43(11), 1999, pp. 2798-2800
Citations number
13
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
43
Issue
11
Year of publication
1999
Pages
2798 - 2800
Database
ISI
SICI code
0066-4804(199911)43:11<2798:OOEBTR>2.0.ZU;2-Y
Abstract
The contribution of the dosage of target enzyme P-450 14 alpha-demethylase (14 alpha DM) to fluconazole resistance in both Candida albicans and Saccha romyces cerevisiae remains unclear. Here, we show that overexpression of Sa ccharomyces P-450 14 alpha DM in S. cerevisiae, under the control of the re gulatable promoter GAL1, results in azole resistance.