Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to HeLa cells after resuscitation
Jm. Cappelier et al., Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to HeLa cells after resuscitation, APPL ENVIR, 65(11), 1999, pp. 5154-5157
The existence of a viable but nonculturable (VBNC) state has been described
for Campylobacter jejuni as it had been for a number pathogenic bacteria.
Three C. jejuni human isolates were suspended in surface water and subseque
ntly entered the VBNC state. After starvation for 30 days, VBNC cells were
inoculated in the yolk sacs of embryonated eggs. Culturable cells were dete
cted in a large proportion of the embryonated eggs inoculated with VBNC C.
jejuni cells. Recovered cells kept their adhesion properties.