Production of the recombinant hIL-4 delta 2, a native isoform of the humaninterleukin-4, in Escherichia coli cells

Authors
Ptitsyn, LR Smirnov, SV Al'tman, IB Samsonova, NN Khodyakova, AV Vasilenko, RN
Citation
Lr. Ptitsyn et al., Production of the recombinant hIL-4 delta 2, a native isoform of the humaninterleukin-4, in Escherichia coli cells, BIOORG KHIM, 25(8), 1999, pp. 623-629
Citations number
29
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
25
Issue
8
Year of publication
1999
Pages
623 - 629
Database
ISI
SICI code
0132-3423(199908)25:8<623:POTRHD>2.0.ZU;2-G
Abstract
Expression plasmids containing the synthetic gene hil-4 delta 2 was constru cted to produce human interleukin-4 in Escherichia coli cells. Strains TG1( pBTIL-4 delta 2) and BL21(DE3)(pETIL-4 delta 2) produced the recombinant pr otein as inclusion bodies, and its production level was up to 30% of the to tal cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T c ell proliferation, and this effect was enhanced by cyclosporin A.